Resazurin-based assay for screening bacteria for radiation sensitivity
نویسندگان
چکیده
We report a simple and efficient colorimetric method to screen large numbers of bacterial strains for UV- and X-radiation sensitivity. We used reference radiation-sensitive and control strains of Escherichia coli K-12 to compare our colorimetric method to a standard clonogenic plating method. Our colorimetric method was as accurate as the standard method and was superior in terms of savings in supplies and man-hours.
منابع مشابه
Validation of a modified fluorimetric assay for the screening of trichomonacidal drugs.
A fluorimetric microassay that uses a redox dye to determine the viability of the flagellate Trichomonas vaginalis has been optimised to provide a more sensitive method to evaluate potential trichomonacidal compounds. Resazurin has been used in recent years to test drugs against different parasites, including trichomonadid protozoa; however, the reproducibility of these resazurin-based methods ...
متن کاملEvaluation of bacterial survival and phagocyte function with a fluorescence-based microplate assay.
To compare antibacterial function in macrophages from mice deficient in the respiratory burst oxidase or inducible nitric oxide synthase, we developed a fluorescence-based microplate assay of bacterial survival. As bacteria grow, they convert a formulation of resazurin termed AlamarBlue from its nonfluorescent oxidized state to its fluorescent reduced state. The time required to achieve a given...
متن کاملMicrotitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals
The resazurin assay utilising microtitre-plate, described by Drummond and Waigh in 2000, has been modified to achieve more accuracy in the determination of the minimum inhibitory concentration (MIC) values of natural products, including crude extracts, chromatographic fractions or purified compounds against various bacterial strains. This modified resazurin method is simple, sensitive, rapid, r...
متن کاملResazurin microtiter assay for detection of drug resistance and determination of critical concentration of cycloserine resistance of Mycobacterium tuberculosis
We evaluated the feasibility of resazurin microtiter assay (REMA) for detecting cycloserine resistance of Mycobacterium tuberculosis. The cutoff value of the critical concentration of cycloserine resistance in vitro about M. tuberculosis isolates was determined innovatively by drawing the receiver operating characteristic (ROC) curve for the first time. The spending time of REMA ranged from 6 t...
متن کاملمقایسه حساسیت سلول های سفید خون محیطی بیماران مبتلا به سرطان پستان و افراد سالم نسبت به آسیب های ناشی از پرتو گاما با استفاده از آزمون کامت قلیایی
Background and purpose : Ënhanced sensitivity to the chromosome-damaging effects of ionizing radiation is a feature of many cancer-predisposing conditions. Ïn this study we compared the radiation sensitivity of breast cancer patients and healthy volunteers by measuring the levels of initial DNÂ damage in peripheral leukocytes using the comet assay (single-cell alkaline gel electrophoresis). ...
متن کامل